Anti-v-Src; clone 327

Analysis Note

ControlNIH/3T3 cell lysate

Application

Research Sub CategoryImmunological Signaling

Research CategorySignaling

Anti-v-Src Antibody, clone 327 detects level of v-Src & has been published & validated for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

v-Src is the product of the Rous sarcoma virus (RSV) oncogene, and functions as a non-receptor tyrosine kinase belonging to the family of Src proteins. Srcs are typically activated by dimerized cell surface receptors such as integrins, receptor tyrosine kinases, and cytokine receptors. Activated Src kinases phosphorylate the cytoplasmic domains of these receptors enabling interaction between these receptors and their downstream effector molecules. Src proteins are involved in many cellular processs such as apoptosis, immunity, gene expression, cell adhesion, cell migration, and cell transformation. Previous studies have reported that v-Src plays an important role in tumorigenesis.

Immunogen

Recombinant protein corresponding to purified pp60src from bacterial recombinants which direct the synthesis of v-Src.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected v-Src in 10 µg of NIH/3T3 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed